Chitosan as a growth stimulator of moringa (Moringa oleifera L.) under in vitro conditions

Document Type : Original Article


1 Department of Biology, University of Basrah, Iraq

2 Department of Horticulture and Landscape, College of Agriculture, University of Basrah, Iraq



This study was conducted with the aim of moringa tree propagation in the presence of chitosan and some growth regulators using tissue culture technique. The stem nodule explants of moringa tree were cultured on Murashige and Skoog medium supplemented with different combinations of benzyl adenine (0.5, 1, and 1.5 mgL-1) and chitosan (0, 5, and 10 mgL-1) in addition to naphthalene acetic acid at a constant concentration of 0.2 mgL-1 for shoot proliferation. Rooting formation on the formed shoots was investigated using different concentrations of Naphthalene acetic acid (0.5, 1, and 1.5 mgL-1). The results showed that the medium supplemented with 5 mgL-1 chitosan had a significant increase in the response of the stem nodule to the shoot proliferation (78.22%) and shoot numbers (5.12). On the other hand, supplementing the medium with 1 mgL-1 benzyl adenine led to a shoot proliferation response of 68.33% and 4.91 shoots per explant. The combination of 5 mgL-1 chitosan and 1.5 mgL-1 benzyl adenine resulted in the highest response to the proliferation of shoots (91.33%). While the combination of 5 mgL-1 chitosan and 1 mgL-1 benzyl adenine resulted in an increased shoot production with 6.73 shoots per explant. The rooting medium supplemented with 1.5 mgL-1 naphthalene acetic acid was significantly superior in terms of shoots response to root formation (71.70%), number of roots per explant (6.67), and root length (3.50 cm). The current results highlight the advantages of including chitosan in the proliferation and rooting medium of moringa.


Conflict of interest statement

The authors declared no conflict of interest.

Funding statement

The authors declared that no funding was received in relation to this manuscript.

Data availability statement

The authors declared that all related data are included in the article.

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Articles in Press, Accepted Manuscript
Available Online from 22 February 2023
  • Receive Date: 30 November 2022
  • Revise Date: 08 December 2022
  • Accept Date: 21 February 2023
  • First Publish Date: 22 February 2023