Chitosan as a growth stimulator of moringa (Moringa oleifera L.) under in vitro conditions

Document Type : Original Article

Authors

1 Department of Biology, University of Basrah, Iraq

2 Department of Horticulture and Landscape, College of Agriculture, University of Basrah, Iraq

10.30493/das.2023.374878

Abstract

This study was conducted with the aim of moringa tree propagation in the presence of chitosan and some growth regulators using tissue culture technique. The stem nodule explants of moringa tree were cultured on Murashige and Skoog medium supplemented with different combinations of benzyl adenine (0.5, 1, and 1.5 mgL-1) and chitosan (0, 5, and 10 mgL-1) in addition to naphthalene acetic acid at a constant concentration of 0.2 mgL-1 for shoot proliferation. Rooting formation on the formed shoots was investigated using different concentrations of Naphthalene acetic acid (0.5, 1, and 1.5 mgL-1). The results showed that the medium supplemented with 5 mgL-1 chitosan had a significant increase in the response of the stem nodule to the shoot proliferation (78.22%) and shoot numbers (5.12). On the other hand, supplementing the medium with 1 mgL-1 benzyl adenine led to a shoot proliferation response of 68.33% and 4.91 shoots per explant. The combination of 5 mgL-1 chitosan and 1.5 mgL-1 benzyl adenine resulted in the highest response to the proliferation of shoots (91.33%). While the combination of 5 mgL-1 chitosan and 1 mgL-1 benzyl adenine resulted in an increased shoot production with 6.73 shoots per explant. The rooting medium supplemented with 1.5 mgL-1 naphthalene acetic acid was significantly superior in terms of shoots response to root formation (71.70%), number of roots per explant (6.67), and root length (3.50 cm). The current results highlight the advantages of including chitosan in the proliferation and rooting medium of moringa.

Keywords

Conflict of interest statement

The authors declared no conflict of interest.

Funding statement

The authors declared that no funding was received in relation to this manuscript.

Data availability statement

The authors declared that all related data are included in the article.

References

  1. Poteet MD, Number U. Biodiesel crop implementation in Hawaii. Honolulu: Prepared by the Hawaii Agriculture Research Center. Aiea, HI for State of Hawaii Department of Agriculture. 2006:89.
  2. Fozia F, Meenu R, Avinash T, Abdul AK, Shaila F. Medicinal properties of Moringa oleifera: An overview of promising healer. J. Med. Plant Res. 2012;6(27):4368-74. DOI
  3. Yadav R, Khare RK, Singhal A. Qualitative phytochemical screening of some selected medicinal plants of shivpuri district (mp). Int. j. life-sci. sci. res. 2017;3(1):844-7. DOI
  4. Abdulkarim SM, Long K, Lai OM, Muhammad SK, Ghazali HM. Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chem. 2005;93(2):253-63. DOI
  5. Ibrahim M. Role of Endogenous and Exogenous Hormones in Bioactive Compounds Production in Medicinal Plants Via In Vitro Culture Technique. In Plant Hormones: Recent Advances, New Perspectives and Applications. Intechopen. 2022:131. DOI
  6. Colunga Biancatelli RM, Berrill M, Catravas JD, Marik PE. Quercetin and vitamin C: an experimental, synergistic therapy for the prevention and treatment of SARS-CoV-2 related disease (COVID-19). Front. Immunol. 2020:1451. DOI
  7. Chibu H, Shibayama H. Effects of chitosan applications on the growth of several crops. In Chitin and Chitosan in Life Science. Kodansha Scientific LTD. 2001;892:305-16.
  8. Charoenwattana PI, Petprapai UM. Effects of chitosan and Lotus extracts as growth promoter in Dendrobium orchid. Int. J. Environ. Rural Dev. 2013;4(2):133-7. DOI
  9. Youssef SM. Chitosan and thidiazuron improve regeneration efficiency of strawberry (Fragaria x ananassa) cv. Festival from different explant types. Middle East J. 2016;5(4):856-67.
  10. Safana HS, Ibrahim MA, Abd AM. Impact of chitosan and benzyl adenine on shoot multiplication of kumquat plant (Citrus japonica) in vitro. Int. J. Agricult. Stat. Sci. Vol. 2022;18(1):359-65.
  11. Sopalun K, Thammasiri K, Ishikawa K. Effects of chitosan as the growth stimulator for Grammatophyllum speciosum in vitro Int. J. Biotechnol. Bioeng. 2010;4(11):828-30. DOI
  12. Dastjerd ZH, Jabbarzadeh Z, Marandi RJ. Interaction effects of chitosan, benzyladenine, and gibberellic acid on in vitro proliferation of M26 apple rootstock. Hortic. Environ. Biotechnol. 2013;54(6):538-47. DOI
  13. Fatima H, Perveen A, Qaiser M. Micropropagation to rescue endangered plant Moringa concanensis Nimmo (Moringaceae). Pak. J. Bot. 2016;48(1):291-4.
  14. Alshwerf AO, Amin LS, Ibrahim F, Youssef J. Protective Effect of Moringa oleifera Extract on Experimentally LPS-induced periodontitis. Int. J. Adv. Res. 2017;5:1734-40. DOI
  15. Drisya Ravi RS, Siril EA, Nair BR. The effect of silver nitrate on micropropagation of Moringa oleifera an important vegetable crop of tropics with substantial nutritional value. Physiol. Mol. Biol. Plants. 2019;25(5):1311-22. DOI
  16. Khierallah HS, Al-Obaidy OM. Effect of explant type and some plant growth regulators on culture initiation of stevia plants in vitro. Iraqi J. Agric. Sci. 2017;48(5):1206-14. DOI
  17. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 1962;15(3):473-97. DOI
  18. Al-Rawi KM, Khalaf Allah AM. Design and analysis of agricultural experiments. El Mousel Univ., Iraq. 2000;19:487.
  19. Palpandi C, Shanmugam V, Shanmugam A. Extraction of chitin and chitosan from shell and operculum of mangrove gastropod Nerita (Dostia) crepidularia Int. J. Med. Med. Sci. 2009;1(5):198-205.
  20. Khalifa NS. Using tissue culture technology in palm propagation. King Abdulaziz Library for Technical Sciences, National Center for Agricultural Technology, King Abdulaziz City for Technical Sciences, Saudi Arabia. 2011:56.
  21. Ibrahim MA, Al-Taha HA, Saaid ZA. Propagation of strawberry via in vitro adventitious shoot formation technique. Iraqi J. Agric. Sci. 2013;44(1),69-80.
  22. Taiz L, Zeiger E, Møller IM, Murphy A. Plant physiology and development. Sinauer Associates Incorporated; 2015.
  23. Ioio RD, Linhares FS, Scacchi E, Casamitjana-Martinez E, Heidstra R, Costantino P, Sabatini S. Cytokinins determine Arabidopsis root-meristem size by controlling cell differentiation. Curr. Biol.. 2007;17(8):678-82. DOI
  24. Hamad MS, Al-Jubouri MT. Effect of BAA and NAA on the micropropagation of citrus rootstocks in vitro. Euphrates J. Agric. Sci. 2014;6,48-56.
  25. Ibrahim MA, Sabty MZ, Mussa SH. In vitro culture of big-sage (Lantana camara) plant. Acta Sci. Pol. Hortorum Cultus. 2020;19(2),67-73. DOI
  26. Ibrahim MA, Yassin MM. Direct shoot regeneration by in vitro culture of the gerbera (Gerbera jamesonii Bolus) capitulum explants. Plant Cell Biotechnol. Mol. Biol. 2020;(49&50)31:1-9.
  27. Ibrahim MA, Jasim AM, Abbas MF. Somatic embryogenesis and plantlet regeneration in Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni. Genet. Plant Physiol. 2011;1(3/4):150-4.
  28. Luan LQ, Ha VT, Nagasawa N, Kume T, Yoshii F, Nakanishi TM. Biological effect of irradiated chitosan on plants in vitro. Biotechnol. Appl. Biochem. 2005;41(1):49-57. DOI
DYSONA - Applied Science

Articles in Press, Accepted Manuscript
Available Online from 22 February 2023

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History

  • Receive Date: 30 November 2022
  • Revise Date: 08 December 2022
  • Accept Date: 21 February 2023
  • First Publish Date: 22 February 2023

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