In vitro effects of cyanobacteria (Oscillatoria tenuis) extracellular products on date palm (Phoenix dactylifera L. cv. ‘Barhee’) propagation

Document Type: Original Article

Authors

1 College of Agriculture, University of Basrah, Basrah, Iraq

2 College of Sciences, University of Basrah, Basrah, Iraq.

3 Department of Marine Biology, Marine Science Center, University of Basrah, Basrah, Iraq

Abstract

This study was conducted to investigate the effect of the medium type (half-strength MS medium and MS free medium) and the extracellular products of cyanobacteria (Oscillatoria tenuis) that were grown under photoperiods of 8 or 16 h day-1 on the shoot proliferation and rooting of in vitro ‘Barhee’ date palm ciltures. MS free medium failed to establish half-strength MS medium results, which indicates that the medium could not sustain cultures requirments during shoot proliferation and rooting phase. On the other hand, half-strength MS medium supplemented with extracellular products of O. tenuis grown at 16 h day-1 photoperiod recorded the best results in regards of response to direct organogenesis and rooting.

Keywords


  1. Al-Baker A. Date palm past and present, the new in the cultivation, industry and trade. Al-Ani Press, Baghdad, Iraq. 1972:1085.
  2. Matar AM. Date Palm Cultivation and Production. Dar Al-Hikmah Press. Basrah University, Iraq. 1991:230.
  3. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 1962;15(3):473-97. DOI
  4. Jazinizadeh E, Zarghami R, Majd A, Iranbakhsh A, Tajaddod G. In vitro production of date palm (Phoenix dactylifera L.) cv.'Barhee'plantlets through direct organogenesis. Biological Forum – An International Journal 2015;7(2):566.
  5. Zaccaro MC, Kato A, Zulpa G, Storni MM, Steyerthal N, Lobasso K, Stella AM. Bioactivity of Scytonema hofmanni (Cyanobacteria) in Lilium alexandrae in vitro propagation. Electron. J. Biotechnol. 2006;9(3):211-4. DOI
  6. Banerjee M, Shrivastava S. An improved protocol for in vitro multiplication of Bacopa monnieri (L.). World J. Microbiol. Biotechnol. 2008;24:1355-9. DOI 
  7. Molnár Z, Ördög V. The effect of cyanobacterial compounds on the organogenesis of pea cultured in vitro. Acta Biol. Szeged. 2005;49(1-2):37-8.
  8. Stirk WA, Ördög V, Van Staden J, Jäger K. Cytokinin-and auxin-like activity in Cyanophyta and microalgae. J. Appl. Phycol. 2002;14(3):215-21. DOI
  9. Keerthiga M, Anand SP, Nandagopalan V, Doss A, Senthilkumar SR. In vitro propagation of Wedelia trilobata (L.) using Phormidium subincrustatum extracts: A novel approach. Asia Pac J Mol Biol Biotechnol. 2012;20(4):147-52.
  10. Mehta J, Kumar V, Syedy M, Upadhyay D, Ansari R, Bisht V, Naaz H, Soni P. In vitro shoot regeneration of Bacopa monnieri (L.) using cyanobacterial media—a novel approach and effect of phytoregulators on in vitro micropropagation. Asian J Plant Sci Res. 2012;2:699-706.
  11. Al-Rawi KM, Khalaf Allah AM. Design and Analysis of Agricultural Experiments. Dar Al Kutb for Printing and Publishing, University of Mosul. 2000:488.
  12. Shanab S, Saker MM, Abdel-Rahman MH. Crude extracts of some fresh water Cyanobacteria have auxin like activity on potato tissue culture. Arab J. Biotechnol. 2003;6(2):297-312.
  13. Al-Mayahi AM. Thidiazuron-induced in vitro bud organogenesis of the date palm (Phoenix dactylifera L.) cv. Hillawi. Afr. J. Biotechnol. 2014;13(35):3581-90 DOI
  14. Manickavelu A, Nadarajan N, Ganesh SK, Ramalingam R, Raguraman S, Gnanamalar RP. Organogenesis induction in rice callus by cyanobacterial extracellular product. Afr. J. Biotechnol. 2006;5(5):437-9.
  15. Seema T, Arnold R, Tiwari A, Mishra RM, Chauhan UK. Cyanobacterial extract and MS media as a novel tool for in vitro regeneration of Stevia rebaudiana Bertoni. J. algal biomass util. 2011;2(2):24-40.
  16. Ghasolia B, Shandilya D, Maheshwari R. Multiple shoot regeneration of Bacopa monnieri (L.) using cyanobacterial media—a novel approach and effect of phytoregulators on in vitro micropropagation. Int. J. Recent Biotechnol. 2013;1(2):27-33.

Articles in Press, Accepted Manuscript
Available Online from 14 October 2020
  • Receive Date: 04 September 2020
  • Revise Date: 08 October 2020
  • Accept Date: 09 October 2020