Evaluation of tetraploid induction in forage sorghum cultivar “Omid-Bakhsh” using colchicine treatment

Document Type: Original Article


Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran


Polyploidy induction can be used as a method to improve forage quality and crop yield. Additionally, autopolyploidy may play a key role in tolerance pathways of biotic and abiotic stresses, such as nutrient deficiency, drought, extreme temperatures, pests, and pathogens. Colchicine is widely used as a polyploidy inducer in plant species. To examine this ability in forage sorghum, the shoot meristems of four-day-old seedlings of “Omid-Bakhsh” sorghum cultivar were treated with colchicine. Three levels of colchicine concentration (0, 0.01, 0.1, and 0.2) and four-time periods (4, 8, 16, and 24 hours) were used. Morphological and cytological properties of treated and non-treated plants were assessed. Results showed that the polyploidy plants had a lesser height, leaf length and number of stomata compared to the normal plants; however, leaf width and thickness, stem diameter, pollen diameter, as well as stomatal length and width were significantly higher in polyploidy plants. On the other hand, there was no significant difference between treated plants and control in regards to the number of leaves.  Furthermore, the highest probability of polyploidy induction rate in “Omid-Bakhsh” cultivar was observed at 0.2% colchicine concentration and eight hours treatment as revealed by flow cytometry data. Protein analysis results showed a significant increase in protein content of polyploidy plants compared to control.


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